Article
The novel mutation K87E in ribosomal protein S12 enhances protein synthesis activity during the late growth phase in Escherichia coli.
Molecular genetics and genomics : MGG - 1 Apr 2004
Hosaka T, Tamehiro N, Chumpolkulwong N, Hori-Takemoto C, Shirouzu M, Yokoyama S, Ochi K
Abstract excerpt
Resistance to streptomycin in bacterial cells often results from a mutation in the rpsL gene that encodes the ribosomal protein S12. We found that a particular rpsL mutation (K87E), newly identified in Escherichia coli, causes aberrant protein synthesis activity late in the growth phase. While protein synthesis decreased with age in cells in the wild-type strain, it was sustained at a high level in the mutant, as...
Topics
- Chloramphenicol O-Acetyltransferase
- Drug Resistance, Microbial
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Green Fluorescent Proteins
- Luminescent Proteins
- Magnesium
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Poly U