Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers.

doi:10.1016/0378-1119(94)90776-5

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers.

Gene - 28 Jan 1994

Metcalf W W, Jiang W, Wanner B L

Abstract excerpt

Escherichia coli hosts were constructed for maintenance of vectors containing the gamma replication origin of the R6K plasmid (oriRR6K gamma) at different copy numbers (15 or 250/cell). Such vectors require the trans-acting II protein (the pir gene product) for replication. New hosts carry pir+ or pir-116 on the chromosome within uidA, the E. coli gene encoding beta-glucuronidase. They were made using the rep...

Topics

Alleles
Bacteriophage M13
Base Sequence
Chromosomes, Bacterial
Cloning, Molecular
Conjugation, Genetic
DNA Helicases
DNA Primers
DNA Replication
DNA-Binding Proteins
Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genes, Bacterial

Genetic Vectors

Genotype

Glucuronidase

Molecular Sequence Data

Mutagenesis, Insertional

Peptide Initiation Factors

Source

Open in PubMed

Identifiers

PMID: 8125283
DOI: 10.1016/0378-1119(94)90776-5

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8125283-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers.PMID 8125283Read ↗

Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria.Semantic proximity Read ↗

Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement.Semantic proximity Read ↗

Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria.Semantic proximity Read ↗

A set of temperature sensitive-replication/-segregation and temperature resistant plasmid vectors with different copy numbers and in an isogenic background (chloramphenicol, kanamycin, lacZ, repA, par, polA).Semantic proximity Read ↗

A natural mutant of plasmid RP4 that confers phage resistance and reduced conjugative transfer.Semantic proximity Read ↗

Replication of the R6K gamma origin in vitro: dependence on wt pi and hyperactive piS87N protein variant.Semantic proximity Read ↗

A Replicative Plasmid Vector Allows Efficient Complementation of Pathogenic Leptospira StrainsSemantic proximity Read ↗

Construction and analysis of F plasmid traR, trbJ, and trbH mutants.Semantic proximity Read ↗

New pSC101-derivative cloning vectors with elevated copy numbers.Semantic proximity Read ↗

Escherichia coli K12 relA strains as safe hosts for expression of recombinant DNA.Semantic proximity Read ↗

Characterization of the growth inhibition phenotype of the kilAtelAB operon from IncP alpha plasmid RK2Ter.Semantic proximity Read ↗

Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.