Escherichia coli K12 relA strains as safe hosts for expression of recombinant DNA.

doi:10.1007/BF00171951

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Article

Escherichia coli K12 relA strains as safe hosts for expression of recombinant DNA.

Applied microbiology and biotechnology - 1 Jan 1995

Schweder T, Hofmann K, Hecker M

Abstract excerpt

Most Escherichia coli K12 strains survive for a relatively long time outside the laboratory. Under the same conditions the isoallelic E. coli K12 relA mutants die faster because they lack the stringent response. The killing rate is increased by using a plasmid-encoded suicide system consisting of...

Topics

Alkaline Phosphatase
Bacteriophage T7
Biotechnology
Chromosome Mapping
Cloning, Molecular
Containment of Biohazards
DNA, Bacterial
DNA, Recombinant
Escherichia coli
Gene Expression
Genes, Bacterial
Genes, Lethal
Genes, Viral
Microscopy, Electron
Muramidase
Mutation
Phosphates
Plasmids

Source

Open in PubMed

Identifiers

PMID: 7765912
DOI: 10.1007/BF00171951

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7765912-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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