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Article

Escherichia coli K12 relA strains as safe hosts for expression of recombinant DNA.

Applied microbiology and biotechnology - 1 Jan 1995

Schweder T, Hofmann K, Hecker M

Abstract excerpt

Most Escherichia coli K12 strains survive for a relatively long time outside the laboratory. Under the same conditions the isoallelic E. coli K12 relA mutants die faster because they lack the stringent response. The killing rate is increased by using a plasmid-encoded suicide system consisting of the phage T7 lysozyme gene driven by the E. coli alkaline phosphatase gene promoter (phoA). Cells containing this...

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Identifiers

PMID: 7765912
DOI: 10.1007/BF00171951

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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