Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement.

doi:10.1016/0378-1119(93)90691-u

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Article

Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement.

Gene - 15 Jul 1993

Metcalf W W, Wanner B L

Abstract excerpt

Five cassettes carrying uidA, encoding beta-glucuronidase, were made for the construction of insertion mutants with transcriptional fusions to uidA. Three uidA cassettes contain antibiotic-resistance genes, for chloramphenicol (Cm), for kanamycin (Km) and neomycin (Nm), or for streptomycin (Sm) a...

Topics

Alleles
Amino Acid Sequence
Base Sequence
Escherichia coli
Gene Expression
Glucuronidase
Molecular Sequence Data
Mutagenesis, Insertional
Plasmids
Transcription, Genetic
beta-Galactosidase

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