Article
Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen.
Experimental cell research - 1 Mar 1994
Noda A, Ning Y, Venable S F, Pereira-Smith O M, Smith J R
Abstract excerpt
We here describe a rapid and simple expression screen method that has allowed us to isolate cDNAs coding for inhibitors of DNA synthesis from senescent human diploid fibroblasts. The assay involved transient transcriptional overexpression of a gene product encoded by a cDNA in a proliferating cel...
Topics
- Amino Acid Sequence
- Autoradiography
- Base Sequence
- Blotting, Northern
- Cell Division
- Cells, Cultured
- Cellular Senescence
- Chromosome Mapping
- Chromosomes, Human, Pair 6
- Cloning, Molecular
- Cyclin-Dependent Kinase Inhibitor p21
- Cyclins
- DNA
- Fibroblasts
- Humans
- Male
- Methods
- Molecular Sequence Data