Article
Deletion of residues K296-G302 from the slowly-cleared tissue-type plasminogen activator t-PA del (G) leads to partial loss of plasminogen activating activity.
Biologicals : journal of the International Association of Biological Standardization - 1 Sept 1993
Dodd I, Browne M J, Chapman C G, Cronk D, Hastwell C, Wilson S, Robinson J H
Abstract excerpt
Plasmids encoding tissue-type plasminogen activators lacking residues K296-G302 were constructed and were expressed in a Hela cell transient expression system. Conditioned media from the cultures were tested in a number of systems designed to detect function or antigen. Functional assays comprised the chromogenic substrate S2288 and three plasminogen activation assays. All t-PA variants were active in all assays...
Topics
- Binding Sites
- Blotting, Western
- Culture Media, Conditioned
- HeLa Cells
- Humans
- Mutation
- Plasminogen
- Recombinant Proteins
- Sequence Deletion
- Tissue Plasminogen Activator