Article
In-frame linker insertion mutagenesis of yeast transposon Ty1: mutations, transposition and dominance.
Gene - 11 Feb 1994
Monokian G M, Braiterman L T, Boeke J D
Abstract excerpt
A plasmid bearing a GAL1::Ty1 fusion that is competent to transpose was mutagenized by insertion of oligodeoxyribonucleotides that precisely introduce four or five codons semirandomly throughout the plasmid. Approximately one quarter of these resulted in inactivation of transposition; these include inactivating insertions in both the TYA and TYB genes, corresponding to retroviral gag and pol genes. Examples of...
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