Article
In-frame linker insertion mutagenesis of yeast transposon Ty1: phenotypic analysis.
Gene - 11 Feb 1994
Braiterman L T, Monokian G M, Eichinger D J, Merbs S L, Gabriel A, Boeke J D
Abstract excerpt
A plasmid bearing a transpositionally functional GAL1::Ty1 fusion was mutagenized by insertion of four or five codons semirandomly throughout the plasmid. This collection of mutant plasmids was introduced into yeast cells and studied with regard to the properties of the mutant Ty1-encoded protein...
Topics
- Codon
- DNA Transposable Elements
- Mutagenesis, Insertional
- Open Reading Frames
- Phenotype
- Plasmids
- Recombinant Fusion Proteins
- Repetitive Sequences, Nucleic Acid
- Restriction Mapping
- Saccharomyces cerevisiae