Article
Mammalian phosphatidylinositol 3'-kinase induces a lethal phenotype on expression in Schizosaccharomyces pombe; comparison with the VPS34 gene product.
European journal of biochemistry - 1 Feb 1994
Kodaki T, Woscholski R, Emr S, Waterfield M D, Nurse P, Parker P J
Abstract excerpt
The 110-kDa catalytic subunit of the phosphatidylinositol 3'-kinase (p110) is shown to be expressed in Schizosaccharomyces pombe as a functional protein, as judged by the accumulation of 3'-phosphorylated lipids in vivo and the extraction of 3'-kinase activity in vitro. On expression of p110, the cells fail to grow and lose viability. In contrast, while the Saccharomyces cerevisiae protein Vps34p can be expressed...
Topics
- Cell Division
- Chromatography, High Pressure Liquid
- Chromatography, Thin Layer
- Cloning, Molecular
- Culture Media
- Fungal Proteins
- Genes, Fungal
- Lipid Metabolism
- Microscopy, Fluorescence
- Phenotype