Limitations of plasmid complementation test for determination of quinolone resistance due to changes in the gyrase A protein and identification of conditional quinolone resistance locus.

doi:10.1128/AAC.37.12.2588

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Article

Limitations of plasmid complementation test for determination of quinolone resistance due to changes in the gyrase A protein and identification of conditional quinolone resistance locus.

Antimicrobial agents and chemotherapy - 1 Dec 1993

Soussy C J, Wolfson J S, Ng E Y, Hooper D C

Abstract excerpt

Plasmid pJSW101 derived from pUC19 and carrying the wild-type gyrA gene was found to be unstable in HM72, a quinolone-resistant (QR) clinical isolate of Escherichia coli, and resulted in no change in quinolone MICs. MICs determined in the presence of ampicillin to ensure plasmid presence, however...

Topics

4-Quinolones
Aged
Aged, 80 and over
Amino Acid Sequence
Anti-Infective Agents
Base Sequence
Cloning, Molecular
DNA Gyrase
DNA Topoisomerases, Type II
Drug Resistance
False Negative Reactions

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genetic Complementation Test

Humans

Molecular Sequence Data

Mutation

Plasmids

Polymerase Chain Reaction

Transformation, Bacterial

Source

Open in PubMed Open full text

Identifiers

PMID: 8109921
DOI: 10.1128/AAC.37.12.2588
PMCID: PMC192746

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8109921-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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