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Article

A versatile expression vector for the in vitro study of protein-protein interactions: characterization of E47 mutant proteins.

Oncogene - 1 Mar 1994

Hainzl T, Boehm T

Abstract excerpt

Several mutants of the E47 protein, a member of the family of basic/helix-loop-helix (b-HLH) transcriptional regulators, were examined for their ability to homo- and heterodimerize with the protein product of the T-cell oncogene tal-1/SCL. For this purpose, a novel bacterial expression system was developed in which proteins are expressed as fusions appended to glutathione-S-transferase via a thrombin cleavage...

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PMID: 8108132

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A versatile expression vector for the in vitro study of protein-protein interactions: characterization of E47 mutant proteins.PMID 8108132Read ↗

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