A versatile expression vector for the in vitro study of protein-protein interactions: characterization of E47 mutant proteins.

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Article

A versatile expression vector for the in vitro study of protein-protein interactions: characterization of E47 mutant proteins.

Oncogene - 1 Mar 1994

Hainzl T, Boehm T

Abstract excerpt

Several mutants of the E47 protein, a member of the family of basic/helix-loop-helix (b-HLH) transcriptional regulators, were examined for their ability to homo- and heterodimerize with the protein product of the T-cell oncogene tal-1/SCL. For this purpose, a novel bacterial expression system was developed in which proteins are expressed as fusions appended to glutathione-S-transferase via a thrombin cleavage...

Topics

Amino Acid Sequence
Bacteria
Base Sequence
Basic Helix-Loop-Helix Transcription Factors
Cloning, Molecular
DNA
DNA-Binding Proteins
Genetic Vectors
Helix-Loop-Helix Motifs
Humans
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Plasmids

Protein Binding

Proto-Oncogene Proteins

T-Cell Acute Lymphocytic Leukemia Protein 1

TCF Transcription Factors

Transcription Factor 7-Like 1 Protein

Source

Open in PubMed

Identifiers

PMID: 8108132

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8108132-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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