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Article

Wild-type operator binding and altered cooperativity for inducer binding of lac repressor dimer mutant R3.

The Journal of biological chemistry - 29 Apr 1994

Chen J, Alberti S, Matthews K S

Abstract excerpt

Substitution of the C-terminal leucine heptad repeat region of the normally tetrameric lactose repressor by the leucine heptad repeat dimerization domain of GCN4 protein resulted in cell extracts containing protein, designated R3, which behaved as a dimer based on gel retardation analysis of DNA binding (Alberti, S., Oehler, S., von Wilcken-Bergmann, B., and Müller-Hill, B. (1993) EMBO J. 12, 3227-3236). We have...

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PMID: 8175655

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