Article
In vivo characterization of mutants of the bacteriophage f1 gene V protein isolated by saturation mutagenesis.
Journal of molecular biology - 18 Feb 1994
Terwilliger T C, Zabin H B, Horvath M P, Sandberg W S, Schlunk P M
Abstract excerpt
The gene V protein of bacteriophage f1 binds to single-stranded nucleic acids and is essential for propagation of phage f1. We tested the function of gene V protein mutants with single amino acid substitutions in two ways: by the ability of the mutant proteins to support phage growth, and by the ability of the mutant proteins, when expressed at high levels, to inhibit the growth of Escherichia coli. The results...
Topics
- DNA, Single-Stranded
- DNA-Binding Proteins
- Escherichia coli
- Inovirus
- Mutation
- Protein Conformation
- Viral Proteins