Article
Selection and characterization of randomly produced mutants of gene V protein of bacteriophage M13.
European journal of biochemistry - 15 Mar 1992
Stassen A P, Zaman G J, van Deursen J M, Schoenmakers J G, Konings R N
Abstract excerpt
Gene V protein of bacteriophage Ff (M13, f1, fd) is a master regulator of phage DNA replication and phage mRNA translation. It exerts these two functions by binding to single-stranded viral DNA or to specific sequences in the 5' ends of its target mRNAs, respectively. To study the structure/function relationship of gene V protein, M13 gene V was inserted in a phagemid expression vector and a library of missense...
Topics
- Bacteriophages
- DNA Replication
- DNA, Single-Stranded
- DNA, Viral
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Immunoblotting
- Mutation
- Plasmids
- Protein Biosynthesis
- RNA, Messenger