Relationship between in vivo activity and in vitro measures of function and stability of a protein.

doi:10.1021/bi00037a039

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Article

Relationship between in vivo activity and in vitro measures of function and stability of a protein.

Biochemistry - 19 Sept 1995

Sandberg W S, Schlunk P M, Zabin H B, Terwilliger T C

Abstract excerpt

The in vivo activities of mutant proteins are readily measured and can potentially be used to estimate changes in in vitro properties such as stability or function, but this connection has not been rigorously established. Gene V protein is a small protein produced by bacteriophage f1 that binds t...

Topics

Binding Sites
DNA, Single-Stranded
DNA-Binding Proteins
Drug Stability
Genes, Viral
Inovirus
Mutagenesis, Site-Directed
Phenotype
Protein Engineering
Structure-Activity Relationship
Viral Proteins

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