Article
Approaches to predicting effects of single amino acid substitutions on the function of a protein.
Biochemistry - 25 Jun 1991
Zabin H B, Horvath M P, Terwilliger T C
Abstract excerpt
The relative activities of 313 mutants of the gene V protein of bacteriophage f1, assayed in vivo, have been used to evaluate two approaches to predicting the effects of single amino acid substitutions on the function of a protein. First, we tested methods that only depend on the properties of th...
Topics
- Amino Acids
- Binding Sites
- Coliphages
- Escherichia coli
- Mutation
- Sequence Homology, Nucleic Acid
- Solubility
- Viral Proteins