Generation of Chinese hamster ovary cell glycosylation mutants by retroviral insertional mutagenesis. Integration into a discrete locus generates mutants expressing high levels of N-glycolylneuraminic acid.

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Article

Generation of Chinese hamster ovary cell glycosylation mutants by retroviral insertional mutagenesis. Integration into a discrete locus generates mutants expressing high levels of N-glycolylneuraminic acid.

The Journal of biological chemistry - 4 Feb 1994

Hubbard S C, Walls L, Ruley H E, Muchmore E A

Abstract excerpt

Retroviral insertional mutagenesis can both generate somatic cell mutants and pinpoint the genomic locus associated with a mutant phenotype. In the present study, this approach was applied to Chinese hamster ovary cells (CHO) made susceptible to Moloney murine leukemia virus (MoMuLV) infection by...

Topics

Animals
Asparagine
Base Sequence
CHO Cells
Clone Cells
Cricetinae
DNA Primers
Glycoproteins
Glycosylation
Kanamycin Kinase
Molecular Sequence Data
Moloney murine leukemia virus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Insertional

Neuraminic Acids

Oligosaccharides

Phenotype

Phosphotransferases (Alcohol Group Acceptor)

Polymerase Chain Reaction

Source

Open in PubMed

Identifiers

PMID: 8106417

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8106417-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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