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Article

Molecular analysis of three gain-of-function CHO mutants that add the bisecting GlcNAc to N-glycans.

Glycobiology - 1 Jan 2005

Stanley Pamela, Sundaram Subha, Tang Jian, Shi Shaolin

Abstract excerpt

LEC10 Chinese hamster ovary (CHO) cells are gain-of-function mutants that express N-acetylglucosaminyltransferase III (GlcNAc-TIII), the glycosyltransferase that adds the bisecting GlcNAc to complex N-glycans. LEC10 cells are useful for glycosylation engineering of recombinant glycoproteins, including antibody therapeutics, for defining lectin recognition specificities and for determining biological functions of...

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PMID: 15329358
DOI: 10.1093/glycob/cwh136

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