Article
Zidovudine susceptibility testing of human immunodeficiency virus type 1 (HIV) clinical isolates.
Journal of virological methods - 1 Mar 1993
Shafer R W, Kozal M J, Katzenstein D A, Lipil W H, Johnstone I F, Merigan T C
Abstract excerpt
Traditional antiviral susceptibility testing methods using cell lines can be applied to no more than about 30% of clinical HIV isolates (Larder et al., 1989a; Fenyo et al., 1989). We tested the cell-free supernatant from low passage clinical HIV isolates using donor peripheral blood mononuclear c...
Topics
- Base Sequence
- CD4-Positive T-Lymphocytes
- Cells, Cultured
- Dose-Response Relationship, Drug
- Drug Resistance, Microbial
- Genes, Viral
- Genes, pol
- HIV Core Protein p24
- HIV Infections
- HIV-1
- Humans
- Leukocyte Count
- Leukocytes, Mononuclear
- Microbial Sensitivity Tests
- Molecular Sequence Data
- Mutation
- Reproducibility of Results
- Serial Passage