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Article

Genetic and physiological studies of Bacillus subtilis sigma A mutants defective in promoter melting.

Journal of bacteriology - 1 Sept 1994

Rong J C, Helmann J D

Abstract excerpt

The Bacillus subtilis sigA gene encodes the primary sigma factor of RNA polymerase and is essential for cell growth. We have mutated conserved region 2.3 of the sigma A protein to substitute each of seven aromatic amino acids with alanine. Several of these aromatic amino acids are proposed to form a melting motif which facilitates the strand separation step of initiation. Holoenzymes containing mutant sigma...

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Identifiers

PMID: 8071196
DOI: 10.1128/jb.176.17.5218-5224.1994
PMCID: PMC196704

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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