Article
Genetic and physiological studies of Bacillus subtilis sigma A mutants defective in promoter melting.
Journal of bacteriology - 1 Sept 1994
Rong J C, Helmann J D
Abstract excerpt
The Bacillus subtilis sigA gene encodes the primary sigma factor of RNA polymerase and is essential for cell growth. We have mutated conserved region 2.3 of the sigma A protein to substitute each of seven aromatic amino acids with alanine. Several of these aromatic amino acids are proposed to for...
Topics
- Alleles
- Amino Acid Sequence
- Bacillus subtilis
- Bacterial Proteins
- Conserved Sequence
- DNA-Directed RNA Polymerases
- Gene Expression
- Genes, Bacterial
- Isopropyl Thiogalactoside
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Insertional
- Nucleic Acid Denaturation
- Promoter Regions, Genetic
- Sequence Homology, Amino Acid
- Sigma Factor