Article
sigma factor mutations affecting the sequence-selective interaction of RNA polymerase with -10 region single-stranded DNA.
Nucleic acids research - 1 Jul 1997
Huang X, Lopez de Saro F J, Helmann J D
Abstract excerpt
Thesigmasubunit of RNA polymerase determines promoter recognition and catalyzes DNA strand separation. The -35 promoter region is recognized by a helix-turn-helix motif in region 4, while the -10 region is specified, at least in part, by an amphipathic helix in region 2. We have proposed that con...
Topics
- Bacillus subtilis
- Binding, Competitive
- Cross-Linking Reagents
- Cyclic AMP-Dependent Protein Kinases
- DNA, Single-Stranded
- DNA-Directed RNA Polymerases
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Helix-Turn-Helix Motifs
- Mutation
- Promoter Regions, Genetic
- Sigma Factor
- Structure-Activity Relationship
- Substrate Specificity
- Ultraviolet Rays