Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

sigma factor mutations affecting the sequence-selective interaction of RNA polymerase with -10 region single-stranded DNA.

Nucleic acids research - 1 Jul 1997

Huang X, Lopez de Saro F J, Helmann J D

Abstract excerpt

Thesigmasubunit of RNA polymerase determines promoter recognition and catalyzes DNA strand separation. The -35 promoter region is recognized by a helix-turn-helix motif in region 4, while the -10 region is specified, at least in part, by an amphipathic helix in region 2. We have proposed that conserved aromatic residues insigmaregion 2.3 interact with the non-template strand of the -10 element to drive open...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacillus subtilis
Share to Electrophoresis, Polyacrylamide Gel

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Escherichia coli

Share to Mutation

Share to Promoter Regions, Genetic

Share to Structure-Activity Relationship

Share to Substrate Specificity

Share to Ultraviolet Rays

Source

Open in PubMed Open full text

Identifiers

PMID: 9185571
DOI: 10.1093/nar/25.13.2603
PMCID: PMC146786

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

sigma factor mutations affecting the sequence-selective interaction of RNA polymerase with -10 region single-stranded DNA.PMID 9185571Read ↗

rRNA promoter regulation by nonoptimal binding of sigma region 1.2: an additional recognition element for RNA polymerase.Semantic proximity Read ↗

Crystal structure of a sigma 70 subunit fragment from E. coli RNA polymerase.Semantic proximity Read ↗

Base-specific recognition of the nontemplate strand of promoter DNA by E. coli RNA polymerase.Semantic proximity Read ↗

Structural basis for -10 promoter element melting by environmentally induced sigma factors.Semantic proximity Read ↗

Roles for inhibitory interactions in the use of the -10 promoter element by sigma 70 holoenzyme.Semantic proximity Read ↗

The sigma subunit of Escherichia coli RNA polymerase senses promoter spacing.Semantic proximity Read ↗

A coiled-coil from the RNA polymerase beta' subunit allosterically induces selective nontemplate strand binding by sigma(70).Semantic proximity Read ↗

Isolation and characterization of mutations in region 1.2 of Escherichia coli sigma70.Semantic proximity Read ↗

Escherichia coli σ⁷⁰ senses sequence and conformation of the promoter spacer region.Semantic proximity Read ↗

Aromatic amino acids in region 2.3 of Escherichia coli sigma 70 participate collectively in the formation of an RNA polymerase-promoter open complex.Semantic proximity Read ↗

Promoter use by sigma 38 (rpoS) RNA polymerase. Amino acid clusters for DNA binding and isomerization.Semantic proximity Read ↗

Substitutions in the Escherichia coli RNA polymerase sigma70 factor that affect recognition of extended -10 elements at promoters.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.