Article
Effects of amino acid substitutions in the promoter -10 binding region of the sigma A factor on growth of Bacillus subtilis.
Journal of bacteriology - 1 Apr 1993
Chang B Y, Doi R H
Abstract excerpt
On the bases of structural and functional information about the Bacillus subtilis sigma A protein and the techniques of site-directed mutagenesis, we constructed a B. subtilis sigA mutant (DB1005) which grows normally at 37 degrees C but is sensitive to higher temperatures. DNA sequencing analyse...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- Binding Sites
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- Protein Conformation
- Sigma Factor
- Structure-Activity Relationship
- Transformation, Bacterial