Article
Effects of amino acid substitutions in the promoter -10 binding region of the sigma A factor on growth of Bacillus subtilis.
Journal of bacteriology - 1 Apr 1993
Chang B Y, Doi R H
Abstract excerpt
On the bases of structural and functional information about the Bacillus subtilis sigma A protein and the techniques of site-directed mutagenesis, we constructed a B. subtilis sigA mutant (DB1005) which grows normally at 37 degrees C but is sensitive to higher temperatures. DNA sequencing analyses revealed that DB1005 has Ile-198-->Ala and Ile-202-->Ala amino acid substitutions in the alpha-helix of the 2.4...
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