Suppressors of the Ca(2+)-sensitive yeast mutant (csg2) identify genes involved in sphingolipid biosynthesis. Cloning and characterization of SCS1, a gene required for serine palmitoyltransferase activity.

Abstract excerpt

Suppressor mutations in Saccharomyces cerevisiae that block Ca(2+)-induced death of csg2 mutant cells were investigated. These mutants, called scs mutants (suppressor of Ca2+ sensitivity), fall into seven complementation groups (scs1-scs7). All mutant strains in two of the complementation groups (scs1 and scs2) simultaneously acquire a requirement for 10 mM Ca2+, whereas wild type grow with only trace amounts of...

Topics

• Acyltransferases
• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• Genes, Fungal
• Inositol
• Molecular Sequence Data
• Mutation
• Phenotype
• Saccharomyces cerevisiae