Article
Coordination between fission yeast glucan formation and growth requires a sphingolipase activity.
Genetics - 1 Aug 2001
Feoktistova A, Magnelli P, Abeijon C, Perez P, Lester R L, Dickson R C, Gould K L
Abstract excerpt
css1 mutants display a novel defect in Schizosaccharomyces pombe cell wall formation. The mutant cells are temperature-sensitive and accumulate large deposits of material that stain with calcofluor and aniline blue in their periplasmic space. Biochemical analyses of this material indicate that it consists of alpha- and beta-glucans in the same ratio as found in cell walls of wild-type S. pombe. Strikingly, the...
Topics
- Amino Acid Sequence
- Cell Division
- Cell Membrane
- Cell Wall
- Cloning, Molecular
- Epitopes
- Gene Deletion
- Glucans
- Immunoblotting
- Microscopy, Electron
- Microscopy, Fluorescence
- Models, Biological
- Models, Genetic
- Molecular Sequence Data