Improved single-strand conformation polymorphism analysis by asymmetric polymerase chain reaction with end-labeled primers.

doi:10.1016/1050-3862(94)90006-x

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Article

Improved single-strand conformation polymorphism analysis by asymmetric polymerase chain reaction with end-labeled primers.

Genetic analysis, techniques and applications - 1 Jan 1994

Russell G C

Abstract excerpt

An improved form of single-strand conformation polymorphism (SSCP) assay has been developed for the analysis of bovine major histocompatibility complex class II alleles. The method uses asymmetric polymerase chain reaction (PCR) amplification from each of two end-labeled primers to generate indiv...

Topics

Alleles
Animals
Base Sequence
Cattle
DNA Primers
DNA, Single-Stranded
Exons
Genes, MHC Class II
Histocompatibility Antigens Class II
Molecular Sequence Data
Nucleic Acid Conformation
Polymerase Chain Reaction
Polymorphism, Genetic

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