Article
Detection of nucleotide variations in the D-loop region of bovine mitochondrial DNA using polymerase chain reaction-based methodologies.
Animal genetics - 1 Oct 1996
Eledath F M, Hines H C
Abstract excerpt
Allele-specific polymerase chain reaction (ASPCR) and single-strand conformation polymorphism (SSCP) were used to characterize 16 Holstein maternal lines. ASPCR was used to detect polymorphic nucleotides at eight different positions in the displacement loop (D-loop) of bovine mitochondrial DNA (m...
Topics
- Animals
- Base Sequence
- Cattle
- DNA, Mitochondrial
- Female
- Gene Frequency
- Genetic Variation
- Genotype
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Reproducibility of Results