A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification.

doi:10.1111/j.1365-2052.1993.tb00253.x

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Article

A polymorphism in the bovine gamma-S-crystallin gene revealed by allele-specific amplification.

Animal genetics - 1 Apr 1993

Kemp S J, Maillard J C, Teale A J

Abstract excerpt

A polymorphism was detected in the 3' untranslated region of the bovine gamma-S-crystallin gene by direct sequencing of polymerase chain reaction (PCR) products from genomic DNA of an N'Dama bull and a Boran cow. A set of three PCR primers was designed to detect this difference and thus give alle...

Topics

Alleles
Animals
Base Sequence
Cattle
Crystallins
DNA, Single-Stranded
Electrophoresis, Agar Gel
Female
Gene Frequency
Genetic Markers
Male
Molecular Sequence Data
Oligonucleotides
Pedigree
Polymerase Chain Reaction
Polymorphism, Genetic

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