Technical note: DNA typing for ovine MHC DRB1 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

doi:10.3168/jds.S0022-0302(03)73939-3

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Article

Technical note: DNA typing for ovine MHC DRB1 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Journal of dairy science - 1 Oct 2003

Konnai S, Nagaoka Y, Takesima S, Onuma M, Aida Y

Abstract excerpt

The ovine major histocompatibilty complex (Ovar) class II DRB1 second exon was amplified by polymerase chain reaction (PCR) from DNA samples of 52 Suffolk sheep. Polymerase chain reaction products were characterized by the restriction fragment length polymorphism (RFLP) technique using nine restriction enzymes, RsaI, HaeIII, SacI, SacII, DdeI, NciI, Hin1I, EcoRI, and BstNI, yielding 13 types. Sequencing of cloned...

Topics

Alleles
Animals
DNA
DNA Restriction Enzymes
Genotype
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Sheep

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Open in PubMed

Identifiers

PMID: 14594256
DOI: 10.3168/jds.S0022-0302(03)73939-3

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