Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Technical note: DNA typing for ovine MHC DRB1 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Journal of dairy science - 1 Oct 2003

Konnai S, Nagaoka Y, Takesima S, Onuma M, Aida Y

Abstract excerpt

The ovine major histocompatibilty complex (Ovar) class II DRB1 second exon was amplified by polymerase chain reaction (PCR) from DNA samples of 52 Suffolk sheep. Polymerase chain reaction products were characterized by the restriction fragment length polymorphism (RFLP) technique using nine restriction enzymes, RsaI, HaeIII, SacI, SacII, DdeI, NciI, Hin1I, EcoRI, and BstNI, yielding 13 types. Sequencing of cloned...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to DNA
Share to DNA Restriction Enzymes

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genotype

Share to Molecular Sequence Data

Share to Polymerase Chain Reaction

Share to Polymorphism, Restriction Fragment Length

Source

Identifiers

PMID: 14594256
DOI: 10.3168/jds.S0022-0302(03)73939-3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Technical note: DNA typing for ovine MHC DRB1 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).PMID 14594256Read ↗

Restriction fragment length polymorphism of DQB and DRB class II genes of the ovine major histocompatibility complex.Semantic proximity Read ↗

Sequence-based genotyping of the sheep MHC class II DRB1 locus.Semantic proximity Read ↗

Genomic organisation and allelic diversity within coding and non-coding regions of the Ovar-DRB1 locus.Semantic proximity Read ↗

A BglII RFLP at the ovine MHC class II DRA locus.Semantic proximity Read ↗

Sequences and diversity of 17 new Ovar-DRB1 alleles from three breeds of sheep.Semantic proximity Read ↗

Restriction fragment length polymorphism of exon 2 Ovar-DRB1 gene in Polish Heath Sheep and Polish Lowland Sheep.Semantic proximity Read ↗

Identification and phylogenetic analysis of 15 MHC class II DRB1 beta1 expressed alleles in a ewe-lamb flock.Semantic proximity Read ↗

Documentation of extensive genetic diversity in the Ovar-DRB1 gene in native Turkish sheep.Semantic proximity Read ↗

Isolation, characterization and evolution of ovine major histocompatibility complex class II DRA and DQA genes.Semantic proximity Read ↗

Allelic polymorphism in the ovine DQA1 gene.Semantic proximity Read ↗

A PCR-RFLP typing method for the caprine Mhc class II DRB gene.Semantic proximity Read ↗

Association of Ovar-DRB1 alleles with innate immune responses in sheep.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.