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Article

Asymmetric single-strand conformation polymorphism: an accurate and cost-effective method to amplify and sequence allelic variants.

American journal of botany - 1 Jul 2011

Rodríguez Flor, Cai Danying, Teng Yuanwen, Spooner David

Abstract excerpt

PREMISE OF THE STUDY: An efficient alternative strategy to conventional cloning was needed to generate high-quality DNA sequences from a variety of nuclear orthologs for phylogenetic studies. This method would facilitate studies and minimize technical problems typically encountered in cloning methodologies. METHODS: We tested a variety of single-strand conformation polymorphism (SSCP) protocols including purified...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 21730333
DOI: 10.3732/ajb.1000251

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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