Use of a single-strand conformation polymorphism analysis to perform a simple genotyping of bovine kappa-casein A and B variants.

Abstract excerpt

We propose an alternative method for casein genotyping, generally carried out using polymerase chain reaction followed by restriction fragment length polymorphism analysis. Application of the single-strand conformation polymorphism technique detects nucleotide changes in the fragment amplified by means of polymerase chain reaction and thus avoids the use of restriction enzymes. A 453 bp fragment from exon IV of...

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