Article
ABO genotyping by single strand conformation polymorphism--using CE.
Electrophoresis - 1 Jul 2009
Lee James Chun-I, Hsieh Hsing-Mei, Teng Hsiao-Feng, Lo Sheng-Chiang, Linacre Adrian, Tsai Li-Chin
Abstract excerpt
This study describes a novel method of ABO genotyping using amplicons generated by PCR-SSCP and separated by CE. We describe four amplicons with sizes of 112, 121, 123 and 160 bp based on SSCP analysis. These amplicons are amplified from exons 6 and 7 and include polymorphic sites of cDNA 261, 297, 467, 526, 646, 657, 681, 703, 1061 and 1096 generated from the ABO gene. The positions of the SSCP alleles, when...
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