Article
Molecular genetic analysis of flow-sorted ovarian tumour cells: improved detection of loss of heterozygosity.
British journal of cancer - 1 Aug 1994
Abeln E C, Corver W E, Kuipers-Dijkshoorn N J, Fleuren G J, Cornelisse C J
Abstract excerpt
Detection of loss of heterozygosity (LOH) is usually performed on homogenised tumour specimens. In this type of analysis samples with a low percentage of tumour cells have to be excluded and possible intra-tumour heterogeneity is obscured. In this study we report the application of polymerase cha...
Topics
- Aneuploidy
- Base Sequence
- Cell Separation
- Chromosome Aberrations
- DNA, Neoplasm
- Diploidy
- Female
- Flow Cytometry
- Genetic Carrier Screening
- Genetic Variation
- Humans
- Molecular Sequence Data
- Ovarian Neoplasms
- Polymerase Chain Reaction
- Tumor Cells, Cultured