Article
Role of two nucleotide-binding regions in an N-ethylmaleimide-sensitive factor involved in vesicle-mediated protein transport.
The Journal of biological chemistry - 12 Aug 1994
Sumida M, Hong R M, Tagaya M
Abstract excerpt
N-Ethylmaleimide-sensitive factor (NSF) was originally characterized as the protein that restores in vitro protein transport activity of the Golgi membranes inactivated by N-ethylmaleimide. This protein has two homologous regions, each containing the consensus sequence for nucleotide binding, and possesses ATPase activity. To investigate the role of the two nucleotide-binding regions in NSF, we have replaced two...
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