Article
"Cold-sensitive" mutants of the Lac repressor.
Journal of bacteriology - 1 Mar 2007
Barker Andrew, Oehler Stefan, Müller-Hill Benno
Abstract excerpt
Thirteen of more than 4,000 single-amino-acid-replacement mutants of the Lac repressor, generated by suppression of amber nonsense mutants, were characterized as having a cold-sensitive phenotype. However, when expressed as missense mutations, none of the replacements cause cold sensitivity, implicating the suppression mechanism as being responsible for this phenotype.
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