"Cold-sensitive" mutants of the Lac repressor.

Abstract excerpt

Thirteen of more than 4,000 single-amino-acid-replacement mutants of the Lac repressor, generated by suppression of amber nonsense mutants, were characterized as having a cold-sensitive phenotype. However, when expressed as missense mutations, none of the replacements cause cold sensitivity, implicating the suppression mechanism as being responsible for this phenotype.

Topics

• Amino Acid Sequence
• Bacterial Proteins
• Codon, Nonsense
• Cold Temperature
• Lac Repressors
• Molecular Sequence Data
• Mutation, Missense
• Phenotype
• Repressor Proteins