Article
Spectrum of glycidyl methacrylate-induced mutation in plasmid-Escherichia coli system.
Biomedical and environmental sciences : BES - 1 Mar 1994
Gao H L, Zuo J, Fang F D
Abstract excerpt
In order to characterize the spectrum of mutation induced by glycidyl methacrylate (GMA), the plasmid pBR322 was modified with this mutagen in vitro, transfected into appropriate Escherichia coli host HB101. The mutants were then screened and defined by DNA sequencing. Sequence analysis reveals that GMA induces two classes of mutations: deletion of the mono-, di- or tetra-base or the insertion of mono- or...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA Mutational Analysis
- DNA, Bacterial
- Epoxy Compounds
- Escherichia coli
- Frameshift Mutation
- Methacrylates
- Molecular Sequence Data
- Mutation
- Plasmids