Article
Analysis of the phenotype and the restriction enzyme mapping level of mutations induced by the new mutagen glycidyl methacrylate.
Biomedical and environmental sciences : BES - 1 Jun 1990
Xie D Y, Gao H L, Zuo J, Zhang W, Li Z S, Yang H F, Fang F D
Abstract excerpt
Glycidyl methacrylate (GMA) is a recently recognized chemical mutagen. In order to explore the mutagenicity and mutagenic process of GMA, plasmid pBR322 was used for in vitro binding, mutant screening, and restriction enzyme mapping. The binding between GMA and DNA in vitro has been verified by means of a spectrophotometric method. When pBR322 and GMA-bound pBR322 were used to transform Escherichia coli HB101,...
Topics
- Ampicillin Resistance
- DNA, Bacterial
- Epoxy Compounds
- Escherichia coli
- Methacrylates
- Mutagens
- Mutation
- Phenotype
- R Factors
- Restriction Mapping
- Tetracycline Resistance