Article
[New mutagen, glycidyl methacrylate (GMA) changed restriction enzyme map of plasmid].
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae - 1 Apr 1991
Zuo J
Abstract excerpt
Our present work showed that the mutant TcS ApR of plasmid pBR322 induced by GMA changed restriction enzyme map in the single and double enzyme digestions. Results indicated that the mutant plasmid has lots of mutated sites which are disappeared on some sites and appeared on others in resistance gene region, for example, sites of Bg I, EcoRI, HindIII and HincII, and occurred a new recognition site for HincII...
Topics
- Animals
- Epoxy Compounds
- Methacrylates
- Mice
- Mutation
- Plasmids
- Restriction Mapping
