Article
Induction of mutations by replication of malondialdehyde-modified M13 DNA in Escherichia coli: determination of the extent of DNA modification, genetic requirements for mutagenesis, and types of mutations induced.
Carcinogenesis - 1 Jan 1995
Benamira M, Johnson K, Chaudhary A, Bruner K, Tibbetts C, Marnett L J
Abstract excerpt
The mutagenicity of the lipid peroxidation product, malondialdehyde (MDA), was measured in the lacZ alpha forward mutation assay using a recombinant M13 phage, M13MB102. Single-stranded M13MB102 DNA was reacted with MDA at neutral pH and the modified DNA was transformed into strains of Escherichia coli induced for the SOS response. Increasing concentrations of MDA led to an increase in lacZ alpha-mutations...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.