Article
Leeway and constraints in the forced evolution of a regulatory RNA helix.
The EMBO journal - 1 Jun 1994
Olsthoorn R C, Licis N, van Duin J
Abstract excerpt
The start of the coat protein gene of RNA phage MS2 adopts a well-defined hairpin structure of 12 bp (including one mismatch) in which the start codon occupies the loop position. An earlier expression study using partial MS2 cDNA clones had indicated that the stability of this hairpin is important for gene expression. For every -1.4 kcal/mol increase in stability a 10-fold reduction in coat protein was obtained....
Topics
- Base Composition
- Base Sequence
- Biological Evolution
- Capsid
- DNA, Complementary
- Levivirus
- Models, Genetic
- Molecular Sequence Data
- Nucleic Acid Conformation
- Phenotype
- Point Mutation
- RNA, Viral