Article
Peptide display on live MS2 phage: restrictions at the RNA genome level.
The Journal of general virology - 1 Jul 2001
van Meerten Dico, Olsthoorn Rene C L, van Duin Jan, Verhaert Raymond M D
Abstract excerpt
The potential of the RNA phage MS2 to accommodate extra amino acids in its major coat protein has been examined. Accordingly, a pentapeptide was encoded in the genome as an N-terminal extension. In the MS2 crystal structure, this part of the coat protein forms a loop that extends from the outer surface of the icosahedral virion. At the RNA level, the insert forms a large loop at the top of an existing hairpin....
Topics
- Base Sequence
- Capsid
- DNA Transposable Elements
- Levivirus
- Mutation
- Nucleic Acid Conformation
- Peptides
- RNA, Viral
- Recombinant Proteins