Mutants of the bacteriophage MS2 coat protein that alter its cooperative binding to RNA.

Abstract excerpt

An RNA binding assay measuring cooperative protein binding has been used to evaluate the effects of mutations in the MS2 phage coat protein expected to disrupt capsid assembly. By using the crystal structure of the virus as a guide, six different mutations in the FG loop structure were selected in which hydrophobic residues were replaced with charged residues. Most of these proteins form capsids in Escherichia...

Topics

• Base Sequence
• Capsid
• Capsid Proteins
• Crystallization
• Escherichia coli
• Macromolecular Substances
• Models, Molecular
• Molecular Sequence Data
• Molecular Structure
• Mutation
• Nucleic Acid Conformation