Article
[A new method of nonradioactive labelling of oligonucleotides and their use as allele-specific probes for detecting mutations causing beta-thalassemia].
Molekuliarnaia biologiia - 1 Jan 2000
Lebedeva I V, Ivanovskaia M G, Fedorov A N, Limborskaia S A, Shabarova Z A
Abstract excerpt
We have developed simple and efficient methods for synthesis of biotin and horseradish peroxidase (HRP)-labeled oligonucleotides. Biotinylated oligonucleotides were obtained in quantitative yields, and oligonucleotide conjugates with HRP in 60-80% yields. Allele-specific oligonucleotide probes fo...
Topics
- Alleles
- Base Sequence
- Biotin
- Genetic Carrier Screening
- Globins
- Homozygote
- Horseradish Peroxidase
- Humans
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- beta-Thalassemia