Genotyping of mutations in the beta-globin gene using allele specific hybridization.

doi:10.1007/978-1-59745-538-1_11

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Article

Genotyping of mutations in the beta-globin gene using allele specific hybridization.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009

Poulsen Lena, Petersen Jesper, Dufva Martin

Abstract excerpt

The use of DNA microarrays for genotyping is economically favorable when compared to real-time PCR and DNA sequencing. Here, we demonstrate a DNA microarray-based assay using allele-specific oligonucleotide (ASO) probes for genotyping mutations in the beta-globin gene. The assay makes use of agarose film coated glass slides as substrate, unmodified melting temperature matched ASO probes, target amplification and...

Topics

Alleles
DNA Probes
Genotype
Humans
Mutation
Oligonucleotide Array Sequence Analysis
Reproducibility of Results
Sepharose
beta-Globins

Source

Open in PubMed

Identifiers

PMID: 19381980
DOI: 10.1007/978-1-59745-538-1_11

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