Rapid and practical detection of beta-globin mutations causing beta-thalassemia by fluorescence-based PCR-single-stranded conformation polymorphism analysis.

doi:10.1006/mcpr.1994.1055

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Article

Rapid and practical detection of beta-globin mutations causing beta-thalassemia by fluorescence-based PCR-single-stranded conformation polymorphism analysis.

Molecular and cellular probes - 1 Oct 1994

Takahashi-Fujii A, Ishino Y, Kato I, Fukumaki Y

Abstract excerpt

We report a useful method for daily clinical examination for the diagnosis of thalassemia. We applied a fluorescence-based image analyser to a non-radioisotopic PCR-single-stranded conformation polymorphism (SSCP) analysis to detect mutations in the beta-globin gene. PCR primers were labelled wit...

Topics

Base Sequence
DNA
DNA Mutational Analysis
Ethidium
Exons
Fluorescence
Globins
Humans
Introns
Molecular Sequence Data
Mutation
Nucleic Acid Conformation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymerase Chain Reaction

Polymorphism, Single-Stranded Conformational

Restriction Mapping

Rhodamines

Silver Staining

beta-Thalassemia

Source

Open in PubMed

Identifiers

PMID: 7877634
DOI: 10.1006/mcpr.1994.1055

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7877634-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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