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Rapid and practical detection of beta-globin mutations causing beta-thalassemia by fluorescence-based PCR-single-stranded conformation polymorphism analysis.

Molecular and cellular probes - 1 Oct 1994

Takahashi-Fujii A, Ishino Y, Kato I, Fukumaki Y

Abstract excerpt

We report a useful method for daily clinical examination for the diagnosis of thalassemia. We applied a fluorescence-based image analyser to a non-radioisotopic PCR-single-stranded conformation polymorphism (SSCP) analysis to detect mutations in the beta-globin gene. PCR primers were labelled with rhodamine X and the amplified fragments from the beta-globin gene were resolved by non-denaturing polyacrylamide gel...

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PMID: 7877634
DOI: 10.1006/mcpr.1994.1055

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