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Article

Detection of beta-thalassemia mutations by ASO hybridization of PCR amplified DNA with digoxigenin ddUTP labeled oligonucleotides.

Hemoglobin - 1 Jan 1991

Efremov D G, Dimovski A J, Efremov G D

Abstract excerpt

A simple procedure for nonradioactive labeling of oligonucleotides has recently been developed (1). It consists of 3' end labeling of oligonucleotides with terminal transferase by incorporation of a single digoxigenin labeled dideoxy uridine triphosphate. We used these oligonucleotides for allele specific oligomer hybridization of polymerase chain reaction amplified DNA, followed by an enzyme-linked immunoassay...

Topics

Alleles
Colorimetry
DNA
DNA Mutational Analysis
DNA Nucleotidylexotransferase
Dideoxynucleotides
Digoxigenin
Enzyme-Linked Immunosorbent Assay
Globins
Humans
Nucleic Acid Hybridization

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oligodeoxyribonucleotides

Polymerase Chain Reaction

Thalassemia

Uracil Nucleotides

Source

Identifiers

PMID: 1814858
DOI: 10.3109/03630269109027900

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1814858-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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