Article
TEM beta-lactamase mutants hydrolysing third-generation cephalosporins. A kinetic and molecular modelling analysis.
Journal of molecular biology - 16 Dec 1994
Raquet X, Lamotte-Brasseur J, Fonzé E, Goussard S, Courvalin P, Frère J M
Abstract excerpt
The catalytic properties of six "natural" mutants of the TEM-1 beta-lactamase have been studied in detail, with special emphasis on their activity versus third-generation cephalosporins. On the basis of the recently determined high-resolution structure of the wild-type enzyme, and of the substrat...
Topics
- Aztreonam
- Binding Sites
- Catalysis
- Cefotaxime
- Ceftazidime
- Cefuroxime
- Cephalosporins
- Escherichia coli
- Hydrolysis
- Kinetics
- Mutation
- Penicillins
- Plasmids
- Stereoisomerism
- Substrate Specificity
- beta-Lactamases