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Article

TEM beta-lactamase mutants hydrolysing third-generation cephalosporins. A kinetic and molecular modelling analysis.

Journal of molecular biology - 16 Dec 1994

Raquet X, Lamotte-Brasseur J, Fonzé E, Goussard S, Courvalin P, Frère J M

Abstract excerpt

The catalytic properties of six "natural" mutants of the TEM-1 beta-lactamase have been studied in detail, with special emphasis on their activity versus third-generation cephalosporins. On the basis of the recently determined high-resolution structure of the wild-type enzyme, and of the substrates' structures optimized by the AMI quantum chemistry method, we have attempted to explain the influences of the...

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PMID: 7990143
DOI: 10.1006/jmbi.1994.1756

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