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Article

Mimicking natural evolution in metallo-beta-lactamases through second-shell ligand mutations.

Proceedings of the National Academy of Sciences of the United States of America - 27 Sept 2005

Tomatis Pablo E, Rasia Rodolfo M, Segovia Lorenzo, Vila Alejandro J

Abstract excerpt

Metallo-beta-lactamases (MBLs) represent the latest generation of beta-lactamases. The structural diversity and broad substrate profile of MBLs allow them to confer resistance to most beta-lactam antibiotics. To explore the evolutionary potential of these enzymes, we have subjected the Bacillus cereus MBL (BcII) to a directed evolution scheme, which resulted in an increased hydrolytic efficiency toward...

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Identifiers

PMID: 16172409
DOI: 10.1073/pnas.0503495102
PMCID: PMC1236536

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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