Article
Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP). I. Saturation and alanine-scanning mutagenesis.
Journal of molecular biology - 4 Nov 1994
Niu W, Zhou Y, Dong Q, Ebright Y W, Ebright R H
Abstract excerpt
It has been proposed that the surface loop consisting of amino acid residues 152 to 166 of the catabolite gene activator protein (CAP) of Escherichia coli makes direct protein-protein contact with RNA polymerase at the lac promoter. In this work, we have used targeted saturation mutagenesis of co...
Topics
- Alanine
- Amino Acid Sequence
- Base Sequence
- Computer Simulation
- Cyclic AMP Receptor Protein
- DNA-Directed RNA Polymerases
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Lac Operon
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Plasmids