Article
Identification of a contact between arginine-180 of the catabolite gene activator protein (CAP) and base pair 5 of the DNA site in the CAP-DNA complex.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1990
Zhang X P, Ebright R H
Abstract excerpt
We have used site-directed mutagenesis to replace amino acid 1 of the recognition alpha-helix of the catabolite gene activator protein (CAP), Arg-180, with glycine and with alanine. Substitution of Arg-180 of CAP eliminated specificity between G.C, A.T, C.G, and T.A at base pair 5 of the DNA half...
Topics
- Alanine
- Arginine
- Base Composition
- Base Sequence
- Binding Sites
- Coliphages
- Cyclic AMP Receptor Protein
- DNA
- Escherichia coli
- Glycine
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Protein Binding
- Protein Conformation