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Article

A lethal mutant of the catabolite gene activator protein CAP of Escherichia coli.

Biological chemistry - 1 Oct 1997

Lopata M, Schlieper D, von Wilcken-Bergmann B, Müller-Hill B

Abstract excerpt

The dimeric catabolite gene activator protein (CAP) of Escherichia coli uses its recognition helix to bind with each subunit the DNA sequence motif 5' G-7T-6G-5A-4 3'. It makes a direct amino acid-base contact with E181 and cytosine in position-5' on the reverse strand. While testing mutants of CAP in position 181 for specificity changes, we found that CAP E181Q is lethal in high amounts for the E. coli strains...

Read the complete abstract on PubMed

Topics

Bacterial Proteins
Bacteriophage lambda
Cyclic AMP Receptor Protein
Cytosine
DNA-Binding Proteins
Escherichia coli

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Escherichia coli Proteins

Lac Repressors

Mutagenesis

Mutation

Protein Conformation

Repressor Proteins

Viral Proteins

Viral Regulatory and Accessory Proteins

Source

Identifiers

PMID: 9372184
DOI: 10.1515/bchm.1997.378.10.1153

Provenance

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Source receipt: pubmed, retrieved 7 Sept 2026.

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